Recombinant Human Laminin alpha 5/LAMA5 protein (ab190413)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant Human Laminin alpha 5/LAMA5 protein
See all Laminin alpha 5/LAMA5 proteins and peptides -
Purity
> 90 % SDS-PAGE.
This protein is expressed in E.coli as inclusion bodies. The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGEFIQVFLLGGSRKRVLVRVERAT VYSVEQDNDLELADAYYLGGVPPDQLPPSLRRLFPTGGSVRGCVKGIKAL GKYVDLKRLNTTGVSAGCTADLLVGRAMTFHGHGFLRLALSNVAPLTGNV YSGFGFHSAQDSALLYYRASPDGLCQVSLQQGRVSLQLLRTEVKTQAGFA DGAPHYVAFYSNATGVWLYVDDQLQQMKPHRGPPPELQPQPEGPPRLLLG GLPESGTIYNFSGCISNVFVQRLLGPQRVFDLQQNLGSVNVSTGCAPALQ AQTPGLGPRGLQATARKASRRSRQPARHPACMLPPHLRTTRDSYQFGGSL SSHLEFVGILARHRNWPSLSMHVLPRSSRGLLLFTARLRPGSPSLALFLS NGHFVAQMEGLGTRLRAQSRQRSRPGRWHKVSVRWEKNRILLVTDGARAW SQEGPHRQHQGAEHPQPHTLFVGGLPASSHSSKLP -
Predicted molecular weight
53 kDa including tags -
Amino acids
3027 to 3482 -
Additional sequence information
Corresponds to the C-terminal of extracellular domain. T7-His-TEV cleavage site Tag at N terminal end.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab190413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This product was previously labelled as Laminin alpha 5
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
General Info
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Alternative names
- KIAA0533
- KIAA1907
- LAMA5
see all -
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
Sequence similarities
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab190413 has not yet been referenced specifically in any publications.