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    recombinant-human-lck-protein-ab79626.pdf

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Recombinant human Lck protein (ab79626)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human Lck protein (ab79626)
  • SDS-PAGE - Recombinant human Lck protein (ab79626)
  • Western blot - Recombinant human Lck protein (ab79626)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human Lck protein
    See all Lck proteins and peptides
  • Biological activity

    Specific Activity: 1.96 pmol/min/µg.

  • Purity

    > 90 % SDS-PAGE.
    Affinity purified.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-Lck antibody [Y123] (ab32149)

Specifications

Our Abpromise guarantee covers the use of ab79626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • IMD22
    • LCK
    • Lck p56
    • LCK proto-oncogene, Src family tyrosine kinase
    • LCK_HUMAN
    • Leukocyte C-terminal Src kinase
    • LSK
    • Lymphocyte cell specific protein tyrosine kinase
    • Lymphocyte cell-specific protein-tyrosine kinase
    • Lymphocyte specific protein tyrosine kinase
    • Membrane associated protein tyrosine kinase
    • Oncogene lck
    • P56 LCK
    • p56(LSTRA) protein tyrosine kinase
    • p56-LCK
    • p56lck
    • pp58 lck
    • pp58lck
    • Protein YT16
    • Proto oncogene tyrosine protein kinase LCK
    • Proto-oncogene Lck
    • Protooncogene tyrosine protein kinase LCK
    • T cell specific protein tyrosine kinase
    • T cell-specific protein-tyrosine kinase
    • T lymphocyte specific protein tyrosine kinase p56lck
    • Tyrosine-protein kinase Lck
    • YT 16
    • YT16
    see all
  • Function

    Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • Tissue specificity

    Expressed specifically in lymphoid cells.
  • Involvement in disease

    Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain

    The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • Post-translational
    modifications

    Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Target information above from: UniProt accession P06239 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human Lck protein (ab79626)
    Functional Studies - Recombinant human Lck protein (ab79626)
    Kinase Assay - Specific activity 1.96 pmol/min/µg.
  • SDS-PAGE - Recombinant human Lck protein (ab79626)
    SDS-PAGE - Recombinant human Lck protein (ab79626)
    SDS-PAGE showing ab79626 at approximately 84kDa (3µg).
  • Western blot - Recombinant human Lck protein (ab79626)
    Western blot - Recombinant human Lck protein (ab79626)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab79626? Please let us know so that we can cite the reference in this datasheet.

ab79626 has not yet been referenced specifically in any publications.

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