Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human LCMT1 protein (Active)
See all LCMT1 proteins and peptides
The specific activity was determined to be 480 pmol/min/mg in a methyltransferase assay using PP2Aβ protein substrate.
Purity> 95 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPY IQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAG MDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSE DTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECV LVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQC DLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLE FLDEMELLEQLMRHYCLCWATKGGNELGLKEITY
Molecular weight informationSDS-PAGE molecular weight: ~63kDa
Amino acids1 to 334
TagsGST tag N-Terminus
Additional sequence informationGenBank: NM_016309
Our Abpromise guarantee covers the use of ab268723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
- CGI 68
FunctionMethylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.
Sequence similaritiesBelongs to the methyltransferase superfamily. LCMT family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268723 has not yet been referenced specifically in any publications.