Key features and details
- Expression system: HEK 293 cells
- Purity: >= 87% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human LDL Receptor protein
See all LDL Receptor proteins and peptides
Assay Conditions: 50 µL reaction mix (50 mM HEPES pH 7.4, 100 mM NaCl, 200 µM CaCl2, 0.05% Tween-20, 0.1% BSA, and up to 1500 ng PCSK9) is added to the wells coated with ab198760. Incubate for 2hr. Add antibody against polyhistidine and then incubate 1 hr. Add secondary HRP labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Purity>= 87 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSG DFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKC ISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDP DCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPD CKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCV NVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDN NGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNL EGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTS LIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISR DIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRA IVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLL SGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWT DIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTT LSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVAT QETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQA LGDVAGRGNEKKPSSVRDYKDDDDK
Predicted molecular weight87 kDa including tags
Amino acids22 to 788
TagsDDDDK tag C-Terminus
Additional sequence informationNM_000527.
Our Abpromise guarantee covers the use of ab198760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- LDL R
FunctionBinds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Involvement in diseaseDefects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
Sequence similaritiesBelongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Cellular localizationCell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198760 has not yet been referenced specifically in any publications.