Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µl
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human LDL Receptor protein (Active)
See all LDL Receptor proteins and peptides
Immobilized Human PCSK9, His Tag at 5μg/mL (100 μL/well) can bind ab220570 with a linear range of 8-320 ng/mL.
Purity> 90 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µl
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGG RVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFV CDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGS DEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSD EENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCE GPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSH VCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKC QCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLR NVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPD GLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPV HGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYW VDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEA IFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGC QYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTV RLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAG R
Predicted molecular weight111 kDa
Amino acids22 to 788
Additional sequence informationextracellular domain with Fc fragment of human IgG1 at the C-terminus. Accession # NP_000518.
Our Abpromise guarantee covers the use of ab220570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
- LDL R
FunctionBinds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Involvement in diseaseDefects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).
Sequence similaritiesBelongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Cellular localizationCell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
- Information by UniProt
Immobilized Human PCSK9 (D374Y) (His Tag) at 5µg/mL (100 µL/well) can bind Human LDL R (Fc Tag) with a linear range of 8-320 ng/mL (QC tested).
ab220570 on SDS-PAGE under reducing (lane 1) and no-reducing (lane 2) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. As a result of glycosylation, the reduced protein migrates at 155-175 kDa and the non-reduced protein migrates at 310-350 kDa.
Example of ab220570 bioactivity.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab220570 has been referenced in 1 publication.
- Abdelwahed KS et al. Pseurotin A as a novel suppressor of hormone dependent breast cancer progression and recurrence by inhibiting PCSK9 secretion and interaction with LDL receptor. Pharmacol Res 158:104847 (2020). PubMed: 32438039