Recombinant Human Legumain protein (Tagged) (ab198767)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Legumain protein (Tagged)
See all Legumain proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab198767 was refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFVPIDDPEDGGKHWVVIVAGSN GWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVIN RPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQ DHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEA CESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWM EDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKR KASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRH LDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRT HCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY -
Predicted molecular weight
51 kDa including tags -
Amino acids
18 to 433 -
Additional sequence information
This is the mature protein without the signal peptide expressed with a 29 amino acid N-terminal T7-His-TEV cleavage tag.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, Sucrose and DTT.
General Info
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Alternative names
- AEP
- Asparaginyl endopeptidase
- cysteine 1
see all -
Function
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
Tissue specificity
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
Sequence similarities
Belongs to the peptidase C13 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Lysosome. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab198767 has not yet been referenced specifically in any publications.