Bioactive grade

Recombinant human Leukotriene A4 hydrolase/LTA4H protein (Active) (ab174008)

Description

  • Product name

    Recombinant human Leukotriene A4 hydrolase/LTA4H protein (Active)
    See all Leukotriene A4 hydrolase/LTA4H proteins and peptides
  • Biological activity

    Measured by its ability to cleave the fluorogenic peptide substrate, Arg – 7 – amido – 4 – methylcoumarin (R-AMC).
    The specific activity is >50 pmol/min/µg, as measured under the described conditions.

  • Purity

    > 80 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNL RSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQE IVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDT PSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPC YLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGG PYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISH SWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGEL QNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGG PEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLY SPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQL NEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWED AIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVT AMLVGKDLKVD
    • Predicted molecular weight

      70 kDa including tags
    • Amino acids

      1 to 611
    • Tags

      His tag C-Terminus
    • Additional sequence information

      NP_000886.1

Specifications

Our Abpromise guarantee covers the use of ab174008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as Leukotriene A4 hydrolase

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.

    pH: 7.50
    Constituents: 0.61% Tris, 0.58% Sodium chloride, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 20 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • FLJ17564
    • Leukotriene A(4) hydrolase
    • Leukotriene A-4 hydrolase
    • Leukotriene A4 hydrolase
    • LKHA4_HUMAN
    • LTA-4 hydrolase
    • LTA4
    • LTA4 hydrolase
    • LTA4H
    see all
  • Function

    Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity.
  • Pathway

    Lipid metabolism; leukotriene B4 biosynthesis.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Human LTA4H (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.
  • Reduced SDS-PAGE analysis of ab174008 staining overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 62-72 kDa.

References

ab174008 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174008.
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