Recombinant human LIF protein (ab188058)
- Datasheet
- References
- Protocols
Overview
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Product nameRecombinant human LIF protein
See all LIF proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceSPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF
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Molecular weight20 kDa
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Amino acids23 to 202
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Specifications
Our Abpromise guarantee covers the use of ab188058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.
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Applications
SDS-PAGE
Functional Studies
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Endotoxin level<=1.000 Eu/µg
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Purity>= 98 % SDS-PAGE.
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.
General Info
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Alternative names
- CDF
- Cholinergic Differentiation Factor
- D factor
see all -
FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
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Sequence similaritiesBelongs to the LIF/OSM family.
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Cellular localizationSecreted.
- Information by UniProt
Datasheets and documents
References
ab188058 has not yet been referenced specifically in any publications.
