Bioactive grade

Recombinant human LIF protein (ab188058)

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Description

  • Product name

    Recombinant human LIF protein
    See all LIF proteins and peptides

  • Biological activity

    The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

    <=1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P15018

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF

    • Predicted molecular weight

      20 kDa

    • Amino acids

      23 to 202

Specifications

Our Abpromise guarantee covers the use of ab188058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.

General Info

  • Alternative names

    • CDF
    • Cholinergic Differentiation Factor
    • D factor
    • DIA
    • Differentiation inducing factor
    • differentiation inhibitory activity
    • Differentiation stimulating factor
    • Differentiation-stimulating factor
    • Emfilermin
    • Hepatocyte stimulating factor III
    • HILDA
    • Human interleukin in DA cells
    • Leukemia inhibitory factor
    • LIF
    • LIF_HUMAN
    • Melanoma derived LPL inhibitor
    • Melanoma-derived LPL inhibitor
    • MLPLI
    see all

  • Function

    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

  • Sequence similarities

    Belongs to the LIF/OSM family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human LIF protein (ab188058)
    SDS-PAGE - Recombinant human LIF protein (ab188058)

     SDS PAGE analysis of ab188058 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab188058 has not yet been referenced specifically in any publications.

Publishing research using ab188058? Please let us know so that we can cite the reference in this datasheet.

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