Bioactive grade

Recombinant human LIF protein (ab188058)

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SDS-PAGE - Recombinant human LIF protein (ab188058)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: >= 98% SDS-PAGE
    • Endotoxin level: <= 1.000 Eu/µg
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human LIF protein
      See all LIF proteins and peptides

    • Biological activity

      The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.

    • Purity

      >= 98 % SDS-PAGE.

    • Endotoxin level

      <=1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P15018

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF

      • Predicted molecular weight

        20 kDa

      • Amino acids

        23 to 202

    Specifications

    Our Abpromise guarantee covers the use of ab188058 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.

    General Info

    • Alternative names

      • CDF
      • Cholinergic Differentiation Factor
      • D factor
      • DIA
      • Differentiation inducing factor
      • differentiation inhibitory activity
      • Differentiation stimulating factor
      • Differentiation-stimulating factor
      • Emfilermin
      • Hepatocyte stimulating factor III
      • HILDA
      • Human interleukin in DA cells
      • Leukemia inhibitory factor
      • LIF
      • LIF_HUMAN
      • Melanoma derived LPL inhibitor
      • Melanoma-derived LPL inhibitor
      • MLPLI
      see all

    • Function

      LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

    • Sequence similarities

      Belongs to the LIF/OSM family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human LIF protein (ab188058)
      SDS-PAGE - Recombinant human LIF protein (ab188058)

       SDS PAGE analysis of ab188058 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab188058? Please let us know so that we can cite the reference in this datasheet.

    ab188058 has not yet been referenced specifically in any publications.

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