Recombinant Human LIME protein (Cytoplasmic domain) (denatured) (ab176038)

Overview

  • Product name
    Recombinant Human LIME protein (Cytoplasmic domain) (denatured)
    See all LIME proteins and peptides
  • Protein length
    Protein fragment
  • Description
    Recombinant Human LIME protein (Cytoplasmic domain)

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSCTACRRP EDAVAPRKRA RRQRARLQGS ATAAEASLLR RTHLCSLSKS DTRLHELHRG PRSSRALRPA SMDLLRPHWL EVSRDITGPQ AAPSAFPHQE LPRALPAAAA TAGCAGLEAT YSNVGLAALP GVSLAASPVV AEYARVQKRK GTHRSPQEPQ QGKTEVTPAA QVDVLYSRVC KPKRRDPGPT TDPLDPKGQG AILALAGDLA YQTLPLRALD VDSGPLENVY ESIRELGDPA GRSSTCGAGT PPASSCPSLG RGWRPLPASL P
    • Molecular weight
      31 kDa including tags
    • Amino acids
      28 to 295
    • Tags
      His tag N-Terminus
    • Additional sequence information
      (NP_060276).

Specifications

Our Abpromise guarantee covers the use of ab176038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 80 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • dJ583P15.4
    • FLJ20406
    • Lck interacting membrane protein
    • Lck interacting molecule
    • Lck interacting transmembrane adaptor 1
    • LIME 1
    • LIME1
    • LP8067
    • RP4 583P15.5
    see all
  • Relevance
    LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • Cellular localization
    Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell

Images

  • 15% SDS-PAGE analysis of ab176038 (3µg).

References

ab176038 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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