Recombinant human LIMK1 protein (ab174066)
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- References
- Protocols
Overview
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Product nameRecombinant human LIMK1 protein
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Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIF RPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKE VKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPW SQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARL MVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFS FGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITV RCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRR GESG
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Molecular weight65 kDa including tags
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Amino acids285 to 638
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Tagsproprietary tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab174066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
The specific activity of LIMK1 was determined to be 8 nmol/min/mg.
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Applications
SDS-PAGE
Western blot
Functional Studies
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Purity> 95 % Densitometry.
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FormLiquid
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Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EC 2.7.11.1
- LIM domain kinase 1
- LIM motif-containing protein kinase
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FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
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Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
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Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
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Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
Cellular localizationCytoplasm. Cell projection > growth cone.
- Information by UniProt
Images
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Functional Studies - Recombinant human LIMK1 protein (ab174066)Kinase Assay demonstrating specific activity of ab174066
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SDS-PAGE - Recombinant human LIMK1 protein (ab174066)SDS-PAGE showing ab174066
Datasheets and documents
References
ab174066 has not yet been referenced specifically in any publications.
