Overview

  • Product name
    Recombinant human LIMK1 protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      P53667
    • Species
      Human
    • Sequence
      GSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIF RPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKE VKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPW SQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARL MVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFS FGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITV RCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRR GESG
    • Molecular weight
      65 kDa including tags
    • Amino acids
      285 to 638
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab174066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of LIMK1 was determined to be 8 nmol/min/mg.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity
    > 95 % Densitometry.

  • Form
    Liquid
  • Additional notes

    ab85154 (Human Cofilin full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC 2.7.11.1
    • LIM domain kinase 1
    • LIM motif-containing protein kinase
    • LIMK
    • LIMK-1
    • limk1
    • LIMK1_HUMAN
    see all
  • Function
    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificity
    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in disease
    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localization
    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt

Images

  • Functional Studies - Active human LIMK1 protein fragment (ab174066)
    Functional Studies - Active human LIMK1 protein fragment (ab174066)

    Kinase Assay demonstrating specific activity of ab174066

  • SDS-PAGE - Active human LIMK1 protein fragment (ab174066)
    SDS-PAGE - Active human LIMK1 protein fragment (ab174066)

    SDS-PAGE showing ab174066

References

ab174066 has not yet been referenced specifically in any publications.

Publishing research using ab174066? Please let us know so that we can cite the reference in this datasheet.

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