Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human LIMPII protein
See all LIMPII proteins and peptides
Measured by its binding ability in a functional ELISA.
Immobilized ab191655 at 5 µg/ml (100 µl/well) can bind rhTSP-2/His with a linear range of 0.1 - 5 µg/ml.
Purity> 95 % SDS-PAGE.
Lyophilized from 0.22 µm filtered solution
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceRVFQ KAVDQSIEKK IVLRNGTEAF DSWEKPPLPV YTQFYFFNVT NPEEILRGET PRVEEVGPYT YRELRNKANI QFGDNGTTIS AVSNKAYVFE RDQSVGDPKI DLIRTLNIPV LTVIEWSQVH FLREIIEAML KAYQQKLFVT HTVDELLWGY KDEILSLIHV FRPDISPYFG LFYEKNGTND GDYVFLTGED SYLNFTKIVE WNGKTSLDWW ITDKCNMING TDGDSFHPLI TKDEVLYVFP SDFCRSVYIT FSDYESVQGL PAFRYKVPAE ILANTSDNAG FCIPEGNCLG SGVLNVSICK NGAPIIMSFP HFYQADERFV SAIEGMHPNQ EDHETFVDIN PLTGIILKAA KRFQINIYVK KLDDFVETGD IRTMVFPVMY LNESVHIDKE TASRLKSMIN TT
Predicted molecular weight73 kDa including tags
Amino acids27 to 432
Additional sequence informationLumenal domain fused with Fc fragment of human IgG1 at the C-terminus.
Our Abpromise guarantee covers the use of ab191655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at +4°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituents: 0.61% Tris, 0.75% Glycine
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
- 85 kDa lysosomal membrane sialoglycoprotein
- 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2
FunctionActs as a lysosomal receptor for glucosylceramidase (GBA) targeting.
(Microbial infection) Acts as a receptor for enterovirus 71.
Involvement in diseaseEpilepsy, progressive myoclonic 4, with or without renal failure
Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
Sequence similaritiesBelongs to the CD36 family.
Cellular localizationLysosome membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab191655 has not yet been referenced specifically in any publications.