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    recombinant-human-lipocalin-2-ngal-protein-ab95007.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Recombinant Human Lipocalin-2 / NGAL protein (ab95007)

  • Datasheet
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Western blot - Recombinant Human Lipocalin-2 / NGAL protein (ab95007)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: WB, IP, ELISA

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    Description

    • Product name

      Recombinant Human Lipocalin-2 / NGAL protein
      See all Lipocalin-2 / NGAL proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      Filtered (0.22 µm).
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MPLGLLWLGL ALLGALHAQA QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG

    Associated products

    • Related Products

      • Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)
      • Anti-Lipocalin-2 / NGAL antibody (ab41105)
      • Anti-Lipocalin-2 / NGAL antibody (ab63929)
      • Anti-Lipocalin-2 / NGAL antibody (ab89819)
      • Anti-Lipocalin-2 / NGAL antibody (ab93963)

    Specifications

    Our Abpromise guarantee covers the use of ab95007 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      Immunoprecipitation

      ELISA

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Constituent: PBS

    • Reconstitution
      Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Aliquot reconstituted protein to avoid repeated freezing /thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

    General Info

    • Alternative names

      • 24p3
      • 25 kDa alpha 2 microglobulin related subunit of MMP9
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
      • Alpha 2 microglobulin related protein
      • HGNC:6526
      • HNL
      • Lcn 2
      • Lcn2
      • Lipocalin-2
      • Migration stimulating factor inhibitor
      • MSFI
      • Neutrophil gelatinase associated lipocalin
      • Neutrophil gelatinase associated lipocalin precursor
      • Neutrophil gelatinase-associated lipocalin
      • NGAL
      • NGAL_HUMAN
      • Oncogene 24p3
      • Oncogenic lipocalin 24p3
      • p25
      • Siderocalin
      • siderocalin LCN2
      • SV40 induced 24P3 protein
      • Uterocalin
      see all
    • Function

      Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • Tissue specificity

      Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the calycin superfamily. Lipocalin family.
    • Cellular localization

      Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Target information above from: UniProt accession P80188 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human Lipocalin-2 / NGAL protein (ab95007)
      Western blot - Recombinant Human Lipocalin-2 / NGAL protein (ab95007)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab95007? Please let us know so that we can cite the reference in this datasheet.

    ab95007 has been referenced in 1 publication.

    • Eade CR  et al. HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria. Protein Pept Lett 22:672-80 (2015). PubMed: 25751267

    Customer reviews and Q&As

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