Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Livin protein (Active)
See all Livin proteins and peptides
The specific activity of ab268367 was 30 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.
Purity> 95 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight informationSDS-PAGE molecular weight : ~60kDa
TagsGST tag N-Terminus
Additional sequence informationGenBank: BC014475
Our Abpromise guarantee covers the use of ab268367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Baculoviral IAP repeat containing 7
- Baculoviral IAP repeat containing protein 7
- Baculoviral IAP repeat-containing protein 7
FunctionProtects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
Tissue specificityIsoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
Sequence similaritiesBelongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger.
Cellular localizationNucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268367 has not yet been referenced specifically in any publications.