Recombinant human LTA protein (ab639)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human LTA protein
See all TNF beta proteins and peptides -
Biological activity
The ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.
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Purity
>= 98 % SDS-PAGE.
Sterile filtered Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFL QDGFSLSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH E VQLFSSQYP FHVPLLSSQKMVYPGLQEPW LHSMYHGAAF QLTQGDQL ST HTDGIPHLVL SPSTVFFGAF AL -
Predicted molecular weight
19 kDa
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Description
Recombinant human TNF beta protein
Specifications
Our Abpromise guarantee covers the use of ab639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
The ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.This product was previously labelled as TNF beta
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with 20µl pure water.
General Info
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Alternative names
- LTalpha
- DAMA-25N12.13-004
- DIF
see all -
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab639 has not yet been referenced specifically in any publications.