Recombinant Human Lumican protein (ab134894)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus, T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Lumican protein
See all Lumican proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab134894 was expressed in E. coli as inclusion bodies, refolded using temperature shift inclusion body refolding technology, chromatographically purified and sterile-filtered. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFQYYDYDFPLSIYGQSSPNCAP ECNCPESYPSAMYCDELKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENV TDLQWLILDHNLLENSKIKGRVFSKLKQLKKLHINHNNLTESVGPLPKSL EDLQLTHNKITKLGSFEGLVNLTFIHLQHNRLKEDAVSAAFKGLKSLEYL DLSFNQIARLPSGLPVSLLTLYLDNNKISNIPDEYFKRFNALQYLRLSHN ELADSGIPGNSFNVSSLVELDLSYNKLKNIPTVNENLENYYLEVNQLEKF DIKSFCKILGPLSYSKIKHLRLDGNRISETSLPPDMYECLRVANEVTLN -
Predicted molecular weight
40 kDa including tags -
Amino acids
15 to 338 -
Tags
His tag N-Terminus , T7 tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab134894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.05% DTT, 0.32% Tris HCl, 0.02% EDTA, 3.4% DL-Arginine
Buffer contains: Sodium chloride, Potassium chloride and Glycerol.
General Info
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Alternative names
- Keratan sulfate proteoglycan lumican
- KSPG lumican
- LDC
see all -
Tissue specificity
Cornea and other tissues. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Developmental stage
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab134894 has not yet been referenced specifically in any publications.