Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      QYYDYDFPLSIYGQSSPNCAPECNCPESYPSAMYCDELKLKSVPMVPPGI KYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGRVFSKLKQL KKLHINHNNLTESVGPLPKSLEDLQLTHNKITKLGSFEGLVNLTFIHLQH NRLKEDAVSAAFKGLKSLEYLDLSFNQIARLPSGLPVSLLTLYLDNNKIS NIPDEYFKRFNALQYLRLSHNELADSGIPGNSFNVSSLVELDLSYNKLKN IPTVNENLENYYLEVNQLEKFDIKSFCKILGPLSYSKIKHLRLDGNRISE TSLPPDMYECLRVANEVTLNVDHHHHHH
    • Molecular weight

      37 kDa
    • Amino acids

      19 to 338
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab151646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.
    ab151646 was determined to be >95% pure by SEC-HPLC and reducing SDS-PAGE.
  • Form

    Lyophilised
  • Additional notes

    ab151646 was produced by transfected Human cells.

    The apparent molecular mass of the protein is approximately 60kDa in SDS-PAGE under reducing conditions due to glycosylation.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: 99% Phosphate Buffer, 0.03% EDTA, 0.88% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS.

General Info

  • Alternative names

    • Keratan sulfate proteoglycan lumican
    • KSPG lumican
    • LDC
    • LUM
    • LUM_HUMAN
    • Lumican
    • Lumican precursor
    • SLRR2D
    see all
  • Tissue specificity

    Cornea and other tissues.
  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Developmental stage

    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • Post-translational
    modifications

    Sulfated on tyrosine residue(s).
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

References

ab151646 has not yet been referenced specifically in any publications.

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