Recombinant Human Lumican protein (ab151646)

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Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human Lumican protein
    See all Lumican proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab151646 was determined to be >95% pure by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P51884

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QYYDYDFPLSIYGQSSPNCAPECNCPESYPSAMYCDELKLKSVPMVPPGI KYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGRVFSKLKQL KKLHINHNNLTESVGPLPKSLEDLQLTHNKITKLGSFEGLVNLTFIHLQH NRLKEDAVSAAFKGLKSLEYLDLSFNQIARLPSGLPVSLLTLYLDNNKIS NIPDEYFKRFNALQYLRLSHNELADSGIPGNSFNVSSLVELDLSYNKLKN IPTVNENLENYYLEVNQLEKFDIKSFCKILGPLSYSKIKHLRLDGNRISE TSLPPDMYECLRVANEVTLNVDHHHHHH

    • Predicted molecular weight

      37 kDa

    • Amino acids

      19 to 338

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab151646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Lyophilized

  • Additional notes

    ab151646 was produced by transfected Human cells.

    The apparent molecular mass of the protein is approximately 60kDa in SDS-PAGE under reducing conditions due to glycosylation.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: 99% Phosphate Buffer, 0.03% EDTA, 0.88% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS.

General Info

  • Alternative names

    • Keratan sulfate proteoglycan lumican
    • KSPG lumican
    • LDC
    • LUM
    • LUM_HUMAN
    • Lumican
    • Lumican precursor
    • SLRR2D
    see all

  • Tissue specificity

    Cornea and other tissues.

  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.

  • Developmental stage

    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.

  • Post-translational
    modifications

    Sulfated on tyrosine residue(s).

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab151646? Please let us know so that we can cite the reference in this datasheet.

ab151646 has not yet been referenced specifically in any publications.

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