Recombinant Human Lyn protein (denatured) (ab139777)

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Description

  • Product name

    Recombinant Human Lyn protein (denatured)
    See all Lyn proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P07948

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGCIKSK GKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGI HPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPG NSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADG LCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAF LEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAY IERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIV TYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQ YQQQP

    • Predicted molecular weight

      61 kDa including tags

    • Amino acids

      1 to 512

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab139777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • AA407514
    • EC 2.7.10.2
    • FLJ26625
    • Hck 2
    • JTK 8
    • JTK8
    • Lck/Yes related novel protein tyrosine kinase
    • LYN
    • LYN proto oncogene, Src family tyrosine kinase
    • LYN_HUMAN
    • ONCOGENE LYN
    • p53Lyn
    • p56Lyn
    • Tyrosine protein kinase LYN
    • Tyrosine-protein kinase Lyn
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog
    see all

  • Function

    Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.

  • Tissue specificity

    Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • Domain

    The protein kinase domain plays an important role in its localization in the cell membrane.

  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination is SH3-dependent.

  • Cellular localization

    Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Lyn protein (denatured) (ab139777)
    SDS-PAGE - Recombinant Human Lyn protein (denatured) (ab139777)
    15% SDS-PAGE analysis of ab139777 (3µg).

References

ab139777 has not yet been referenced specifically in any publications.

Publishing research using ab139777? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Has the actiivity of this protein been tested?

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Answer

The laboratory responded that the biological activity for ab139777 has not been tested, and it is unknown when it will be tested. The laboratory suspects that the protein is denatured.

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