Description

  • Product name

    Recombinant Human LYRIC/AEG1 protein (His tag)
  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MKHHHHHHAS VSSGLNENLT VNGGGWNEKS VKLSSQISAG EEKWNSVSPA SAGKRKAEPS AWSQDTGDAN TNGKDWGRSW SDRSIFSGIG STAEPVSQST TSDYQWDVSR NQPYIDDEWS GLNGLSSADP NSDWNAPAEE WGNWVDEERA SLLKSQEPIP DDQKVSDDDK EKGEGALPTG KSKKKKKKKK KQGEDN
    • Predicted molecular weight

      22 kDa including tags
    • Amino acids

      271 to 456
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab206442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    LC-MS/MS
  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as LYRIC

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 0.32% Tris buffer, 0.29% Sodium chloride

  • Reconstitution
    Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

General Info

  • Alternative names

    • 3D3
    • 3D3/LYRIC
    • AEG 1
    • AEG-1
    • AEG1
    • Astrocyte elevated gene 1
    • Astrocyte elevated gene-1 protein
    • LYRIC
    • LYRIC/3D3
    • LYRIC_HUMAN
    • Lysine rich CEACAM1 associated protein
    • Lysine rich CEACAM1 co isolated protein
    • Lysine-rich CEACAM1 co-isolated protein
    • Metadherin
    • Metastasis adhesion protein
    • MTDH
    • Protein LYRIC
    see all
  • Function

    Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
  • Tissue specificity

    Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
  • Cellular localization

    Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
  • Information by UniProt

Images

  • 14 % SDS-PAGE separation of ab206442.

    Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa
    Lane 2: Reduced and boiled sample, 2.5 μg/lane
    Lane 3: Non-reduced and non-boiled sample, 2.5 μg/lane

References

ab206442 has not yet been referenced specifically in any publications.

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