Description

  • Product name

    Recombinant Human MAGE3 protein
  • Purity

    > 90 % SDS-PAGE.
    ab99958 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMPLEQRSQHCKPEEGLEARGEALGLVG AQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNY PLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAR EPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYI FATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSV LEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRA LVETSYVKVLHHMVKISGGPHISYPPLHEWVLREGEE
    • Predicted molecular weight

      37 kDa including tags
    • Amino acids

      1 to 314
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Additional notes

     This product was previously labelled as MAGEA3

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • Antigen MZ2 D
    • Antigen MZ2-D
    • Cancer/testis antigen 1.3
    • Cancer/testis antigen family 1 member 3
    • CT1.3
    • HIP8
    • HYPD
    • MAGA3_HUMAN
    • MAGE 3 antigen
    • MAGE family member A3
    • MAGE-3 antigen
    • MAGE3
    • MAGEA3
    • MAGEA6
    • Melanoma antigen family A 3
    • Melanoma associated antigen 3
    • Melanoma-associated antigen 3
    • MGC1461
    see all
  • Function

    Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab99958 (3µg).

References

ab99958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99958.
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