Recombinant Human MAGI3 protein (ab127432)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His-DHFR tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human MAGI3 protein -
Purity
> 80 % SDS-PAGE.
Purified via His tag -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
29 kDa -
Amino acids
714 to 983 -
Tags
His-DHFR tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab127432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride
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ReconstitutionReconstitute with water to desired concentration.
General Info
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Alternative names
- dJ730K3.2
- MAGI-3
- magi3
see all -
Function
Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains. -
Post-translational
modificationsUbiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex. -
Cellular localization
Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab127432 has not yet been referenced specifically in any publications.