Overview

  • Product name

    Recombinant Human MAP3K12 protein 
  • Protein length

    Protein fragment
  • Description

    Recombinant Human MAP3K12 protein

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      94 kDa including tags
    • Amino acids

      1 to 520

Specifications

Our Abpromise guarantee covers the use of ab131688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity

    > 75 % SDS-PAGE.
    Assessed by densitometry.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • Dlk
    • Dual leucine zipper bearing kinase
    • Dual leucine zipper kinase DLK
    • Leucine-zipper protein kinase
    • M3K12_HUMAN
    • Map3k12
    • MAPK-upstream kinase
    • MEKK12
    • Mitogen-activated protein kinase kinase kinase 12
    • Mixed lineage kinase
    • MUK
    • Protein kinase MUK
    • ZPK
    • ZPKP1
    see all
  • Function

    May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
  • Tissue specificity

    Highly expressed in brain and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    Interacts with MBIP through the leucine-zipper motif.
  • Post-translational
    modifications

    Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt

Images

  • The purity of 131688 was determined to be >75% by densitometry.

References

This product has been referenced in:

  • Cornett EM  et al. A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity. Sci Adv 4:eaav2623 (2018). Read more (PubMed: 30498785) »
See 1 Publication for this product

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