Description

  • Product name

    Recombinant human MAP4K1/HPK1 protein
  • Biological activity

    The Specific activity of ab107953 was determined to be 125 nmol/min/mg.
  • Purity

    > 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      65 kDa including tags
    • Amino acids

      1 to 346

Specifications

Our Abpromise guarantee covers the use of ab107953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

     This product was previously labelled as MAP4K1

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Hematopoietic progenitor kinase
    • Hematopoietic progenitor kinase 1
    • HPK1
    • Human Hematopoietic Progenitor Kinase
    • M4K1_HUMAN
    • Map4k1
    • MAPK/ERK kinase kinase kinase 1
    • MEK kinase kinase 1
    • MEKKK 1
    • Mitogen activated protein kinase kinase kinase kinase 1
    • Mitogen-activated protein kinase kinase kinase kinase 1
    see all
  • Function

    May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
  • Tissue specificity

    Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Information by UniProt

Images

  • The specific activity of MAP4K1/HPK1 (ab107953) was determined to be 110 nmol/min/mg as per activity assay protocol and was equivalent to 144 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab107953
  • SDS PAGE analysis of ab107953
  • SDS-PAGE showing ab107953 at approximately 65kDa.
  • Kinase Assay demonstrating specific activity of ab107953.

References

ab107953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. The tag for this protein is a GST tag. Please let me know if I can be of further assistance.

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