The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
E3 ubiquitin protein ligase MARCH1
E3 ubiquitin-protein ligase MARCH1
Membrane associated RING CH protein I
Membrane associated ring finger (C3HC4) 1
Membrane associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase
Membrane associated RING finger protein 1
Membrane-associated RING finger protein 1
Membrane-associated RING-CH protein I
RING finger protein 171
E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, downregulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B cells, moderate in dendritic cells and low in splenic T cells.
Protein modification; protein ubiquitination.
Contains 1 RING-CH-type zinc finger.
During maturation of dendritic cells, expression is down-regulated and stabilizes MHC class II proteins accumulate at the plasma membrane.
The RING-CH-type zinc finger domain is required for E3 ligase activity.
Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins.
Golgi apparatus > trans-Golgi network membrane. Lysosome membrane. Cytoplasmic vesicle membrane. Late endosome membrane. Early endosome membrane. Cell membrane.