Recombinant human MARK2 protein (ab119135)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Description
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Product name
Recombinant human MARK2 protein -
Biological activity
Specific Activity: 725 - 795 nmol/min/mg
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Purity
> 70 % SDS-PAGE.
Purity was determined to be >70% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
114 kDa including tags -
Amino acids
1 to 788
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab119135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ELKL motif kinase
- ELKL motif kinase 1
- EMK-1
see all -
Function
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
Tissue specificity
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Cellular localization
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
Images
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The specific activity of MARK2 (ab119135) was determined to be 790 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab119135
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SDS PAGE analysis of ab119135
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Sample Kinase Activity Plot: The specific activity of ab119135 was determined to be 795 nmol/min/mg.
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SDS-PAGE analysis of ab119135.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab119135 has not yet been referenced specifically in any publications.