Description

  • Product name

    Recombinant human Mark3 protein
  • Biological activity

    Specific activity 722 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • C-TAK1
    • Cdc25C associated protein kinase 1
    • Cdc25C-associated protein kinase 1
    • cTAK1
    • ELKL motif kinase 2
    • EMK-2
    • Emk2
    • ETK 1
    • KIAA4230
    • KP78
    • MAP microtubule affinity regulating kinase 3
    • MAP/microtubule affinity-regulating kinase 3
    • Mark3
    • MARK3_HUMAN
    • Par 1a
    • PAR1A
    • Protein kinase STK10
    • Ser/Thr protein kinase PAR-1
    • Serine threonine protein kinase p78
    • Serine/threonine-protein kinase p78
    • SerThr protein kinase PAR 1
    see all
  • Function

    Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Information by UniProt

Images

  • The specific activity of Mark3 (ab60012) was determined to be 293.5 nmol/min/mg as per activity assay protocol and was equivalent to 830 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60012
  • Sample Kinase Activity Plot.
  • ab60012 on SDS-PAGE. The extra bands are thought to be truncated MARK3 and tag.

References

ab60012 has not yet been referenced specifically in any publications.

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