Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T
    • Molecular weight

      31 kDa including tags
    • Amino acids

      1 to 261
    • Tags

      His tag N-Terminus
    • Additional sequence information

      ENOPH1 Human Recombinant fused with a20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing281 amino acids (1-261 a.a.).

Specifications

Our Abpromise guarantee covers the use of ab125778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 90 % SDS-PAGE.
    ab125778 was purified by proprietary chromatographic techniques and filter sterilized.
  • Form

    Liquid
  • Additional notes

    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under desiccating conditions.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • 2
    • 2 3 diketo 5 methylthio 1 phosphopentane phosphatase
    • 3-diketo-5-methylthio-1-phosphopentane phosphatase
    • Acireductone synthase
    • DKFZp586M0524
    • E 1 enzyme
    • E1
    • Enolase phosphatase 1
    • Enolase phosphatase E1
    • Enolase-phosphatase E1
    • ENOPH_HUMAN
    • Enoph1
    • FLJ12594
    • MASA homolog
    • MST145
    • MSTP145 protein
    see all
  • Function

    Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
  • Pathway

    Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
    Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
  • Sequence similarities

    Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

References

ab125778 has not yet been referenced specifically in any publications.

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