The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE. ab185595 was expressed in E. coli as inclusion bodies, refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 8.00 Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
3 N(4) ethenocytosine glycosylase
G/5 fluorouracil mismatch glycosylase with biphasic kinetics
G/T mismatch glycosylase
G/U mismatch glycosylase
Methyl CpG binding domain protein 4
Methyl CpG binding endonuclease 1
Methyl CpG binding protein MBD4
Methyl-CpG-binding domain protein 4
Methyl-CpG-binding endonuclease 1
Methyl-CpG-binding protein MBD4
Mismatch specific DNA N glycosylase
Mismatch-specific DNA N-glycosylase
Putative methyl CpG binding protein
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.