Key features and details
- Expression system: Baculovirus
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Product nameRecombinant human MCK10/NEP protein (ab101591)
See all MCK10/NEP proteins and peptides
The specific activity of MCK10/NEP is determined to be 5 nmol/min/mg
Purity> 90 % SDS-PAGE.
The purity was determined to be >90% by densitometry
Protein lengthProtein fragment
Predicted molecular weight77 kDa including tags
Amino acids444 to 876
DescriptionRecombinant human MCK10/NEP protein
Our Abpromise guarantee covers the use of ab101591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204867 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MCK10
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CD 167
FunctionMay be involved in cell-cell interactions and recognition.
Tissue specificityDetected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain.
DomainThe Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
Cellular localizationSecreted and Membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab101591 has not yet been referenced specifically in any publications.