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  1. Link

    recombinant-human-mcl1-protein-ab131682.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Recombinant Human MCL1 protein (ab131682)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human MCL1 protein (ab131682)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

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    Description

    • Product name

      Recombinant Human MCL1 protein
    • Purity

      > 90 % SDS-PAGE.
      ab131682 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      Q07820
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG
      • Predicted molecular weight

        37 kDa including tags
      • Amino acids

        1 to 327
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-MCL1 antibody (ab28147)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab131682 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

    General Info

    • Alternative names

      • Bcl 2 related protein EAT/mcl1
      • Bcl-2-like protein 3
      • Bcl-2-related protein EAT/mcl1
      • BCL2 related
      • Bcl2-L-3
      • BCL2L3
      • EAT
      • Induced myeloid leukemia cell differentiation protein Mcl 1
      • Induced myeloid leukemia cell differentiation protein Mcl-1
      • MCL 1
      • MCL1
      • MCL1-ES
      • mcl1/EAT
      • MCL1_HUMAN
      • MCL1L
      • MCL1S
      • MGC104264
      • MGC1839
      • Myeloid Cell Leukemia 1
      • Myeloid cell leukemia ES
      • Myeloid cell leukemia sequence 1
      • Myeloid cell leukemia sequence 1 BCL2 related
      • Myeloid cell leukemia sequence 1 isoform 1
      • OTTHUMP00000032794
      • OTTHUMP00000032795
      • TM
      see all
    • Function

      Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Post-translational
      modifications

      Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
      Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
      Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
    • Cellular localization

      Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
    • Target information above from: UniProt accession Q07820 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MCL1 protein (ab131682)
      SDS-PAGE - Recombinant Human MCL1 protein (ab131682)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab131682? Please let us know so that we can cite the reference in this datasheet.

    ab131682 has been referenced in 2 publications.

    • D de Araujo A  et al. Bicyclic Helical Peptides as Dual Inhibitors Selective for Bcl2A1 and Mcl-1 Proteins. J Med Chem 61:2962-2972 (2018). PubMed: 29584430
    • Chu R  et al. Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified. Oncotarget 7:78958-78970 (2016). WB, IP ; Human . PubMed: 27738316

    Customer reviews and Q&As

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