Description

  • Product name

    Recombinant Human MCL1 protein
    See all MCL1 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab131682 was purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH VEDLEGG
    • Predicted molecular weight

      37 kDa including tags
    • Amino acids

      1 to 327
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab131682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.32% Tris HCl, 20% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names

    • Bcl 2 related protein EAT/mcl1
    • Bcl-2-like protein 3
    • Bcl-2-related protein EAT/mcl1
    • BCL2 related
    • Bcl2-L-3
    • BCL2L3
    • EAT
    • Induced myeloid leukemia cell differentiation protein Mcl 1
    • Induced myeloid leukemia cell differentiation protein Mcl-1
    • MCL 1
    • MCL1
    • MCL1-ES
    • mcl1/EAT
    • MCL1_HUMAN
    • MCL1L
    • MCL1S
    • MGC104264
    • MGC1839
    • Myeloid Cell Leukemia 1
    • Myeloid cell leukemia ES
    • Myeloid cell leukemia sequence 1
    • Myeloid cell leukemia sequence 1 BCL2 related
    • Myeloid cell leukemia sequence 1 isoform 1
    • OTTHUMP00000032794
    • OTTHUMP00000032795
    • TM
    see all
  • Function

    Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Post-translational
    modifications

    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localization

    Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of 3 µg ab131682.

References

This product has been referenced in:

  • Chu R  et al. Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified. Oncotarget 7:78958-78970 (2016). WB, IP ; Human . Read more (PubMed: 27738316) »
See 1 Publication for this product

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