The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
< 0.100 Eu/µg
hMCP-3 produced a 10-fold increase in chemotaxis at 100 ng/ml compared to the medium control in an assay using differentiated HL-60 cells plated on a primary endothelial cell layer.
The assays were done using microporous cell culture inserts of 3µ pore size that were not protein coated
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
C-C motif chemokine 7
Chemokine CC motif ligand 7
Monocyte chemoattractant protein 3
Monocyte chemotactic protein 3
Small-inducible cytokine A7
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Belongs to the intercrine beta (chemokine CC) family.