Overview

  • Product name

    Recombinant Human MCT-1 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMFKKFDE KENVSNCIQL KTSVIKGIKN QLIEQFPGIE PWLNQIMPKK DPVKIVRCHE HIEILTVNGE LLFFRQREGP FYPTLRLLHK YPFILPHQQV DKGAIKFVLS GANIMCPGLT SPGAKLYPAA VDTIVAIMAE GKQHALCVGV MKMSAEDIEK VNKGIGIENI HYLNDGLWHM KTYK
    • Molecular weight

      23 kDa including tags
    • Amino acids

      1 to 181
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab140541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 95 % SDS-PAGE.
    ab140541 is purified using conventional chromatography techniques.
  • Form

    Liquid
  • Additional notes

    Previously labelled as Malignant T cell amplified sequence 1. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • FLJ39637
    • Malignant T cell amplified sequence 1
    • Malignant T cell-amplified sequence 1
    • MCT 1
    • MCT-1
    • MCT1
    • MCTS 1
    • MCTS1
    • MCTS1_HUMAN
    • Multiple copies T cell malignancies
    • Multiple copies T cell malignancies 1
    • Multiple copies T-cell malignancies
    • Oncogene MCT 1
    • Oncogene MCT1
    see all
  • Function

    Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when over-expressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines over-expressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3.
  • Tissue specificity

    Ubiquitous. Over-expressed in T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines as well as in a subset of primary large B-cell lymphomas.
  • Sequence similarities

    Belongs to the MCTS1 family.
    Contains 1 PUA domain.
  • Domain

    The PUA RNA-binding domain is critical for cap binding, but not sufficient for translation enhancer function. MCT1 N-terminal region is required to enhance translation possibly trough interaction with other proteins.
  • Post-translational
    modifications

    Phosphorylation is critical for stabilization and promotion of cell proliferation.
  • Cellular localization

    Cytoplasm. Nuclear relocalization after DNA damage.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab140541 (3ug)

References

ab140541 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab140541.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up