Recombinant Human MDM2 protein (ab82080)

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Description

  • Product name

    Recombinant Human MDM2 protein
    See all MDM2 proteins and peptides

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAHHHHHHASMCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKS VGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPS FSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQ ELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSIS LSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDS FEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGE ISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQE SEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVI CQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYF PGLEHHHHHHHH

    • Amino acids

      1 to 491

    • Tags

      His tag C-Terminus , His tag N-Terminus

    • Additional sequence information

      This protein has a 6X His tag on its N-Terminus and a 8X His tag on its C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab82080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

General Info

  • Alternative names

    • ACTFS
    • Double minute 2 protein
    • E3 ubiquitin-protein ligase Mdm2
    • Hdm 2
    • Hdm2
    • HDMX
    • MDM 2
    • MDM2
    • MDM2 oncogene E3 ubiquitin protein ligase
    • Mdm2 p53 E3 ubiquitin protein ligase homolog
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa
    • MDM2_HUMAN
    • MDM2BP
    • Mouse Double Minute 2
    • MTBP
    • Murine Double Minute Chromosome 2
    • Oncoprotein Mdm2
    • p53 Binding Protein Mdm2
    • p53-binding protein Mdm2
    • Ubiquitin protein ligase E3 Mdm2
    see all

  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.

  • Tissue specificity

    Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

  • Involvement in disease

    Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.

  • Sequence similarities

    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.

  • Domain

    Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.

  • Post-translational
    modifications

    Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
    Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.

  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
  • Information by UniProt

References

This product has been referenced in:

  • Elshafey R  et al. Label-free impedimetric immunosensor for ultrasensitive detection of cancer marker Murine double minute 2 in brain tissue. Biosens Bioelectron 39:220-5 (2013). Read more (PubMed: 22898660) »
See 1 Publication for this product

Publishing research using ab82080? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Customer contacted us for MDM2 protein sequence ab82080

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Answer


The sequence for the MDM 2 protein (His tag) ab82080 is as follows:

MAHHHHHHAS
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
GLEHHHHHHHH

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Question

Dear Madam or Sir,
I would like to know if the MDM2 protein (His tag) (ab82080) has enzymatic
activity? Can I use it in an in vitro deubiquitination assay?
Thanks a lot!
Best regards,

Read More
Answer

Thank you for your inquiry.

I just went through our database and we do nothave any experimental data about the ligase activity ab82080 (MDM2).

This protein together with p53 wt has beenused byseveral pharma companies and academic labssince 2005 and we have received no complains from these customers.

The protein shall be active although we have little information about the applications customers used itfor.

I am sorry I do not have a more precise answer and hope this information is nevertheless helpful.

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Question

What end of the protein is the His tag on? What is the exact sequence of this MDM2 protei

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Answer

Thank you for your inquiry, and for your patience while I looked into this. The MDM2 protein has a 6x His tag on its N-terminus and 8 x Histag on its C-terminus. The protein sequence is given below: MAHHHHHHAS MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP GLEHHHHHHHH   Please do not hesitate to contact us if we can be of any further assistance to

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Question

I would like to test ab82080 for functional studies involving protein-protein interactions.

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Answer

I am very pleased to hear you would like to accept our offer and test ab82080 in functional studies involving protein-protein interactions. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview for functional studies involving protein-protein interactions and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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