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    recombinant-human-mdm2-protein-ab82080.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Recombinant Human MDM2 protein (ab82080)

  • Datasheet
Submit a review Q&A (4)References (1)

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SDS-PAGE - Recombinant Human MDM2 protein (ab82080)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag C-Terminus, His tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human MDM2 protein
      See all MDM2 proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      ab82080 is purified by affinity and FPLC chromatography and is greater than 95% homogeneous based on SDS-PAGE analysis.
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MAHHHHHHASMCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKS VGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPS FSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQ ELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSIS LSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDS VSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDS FEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGE ISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQE SEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVI CQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYF PGLEHHHHHHHH
      • Predicted molecular weight

        58 kDa
      • Amino acids

        1 to 491
      • Tags

        His tag C-Terminus , His tag N-Terminus
      • Additional sequence information

        This protein has a 6X His tag on its N-Terminus and a 8X His tag on its C-Terminus

    Associated products

    • Related Products

      • Anti-MDM2 (phospho S185) antibody (ab1094)
      • Anti-MDM2 antibody [2A10] (ab16895)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-MDM2 antibody [SMP 14] (ab3110)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab82080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      1 unit equals 1 nanogram of purified protein.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.9
      Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

    General Info

    • Alternative names

      • ACTFS
      • Double minute 2 protein
      • E3 ubiquitin-protein ligase Mdm2
      • Hdm 2
      • Hdm2
      • HDMX
      • MDM 2
      • MDM2
      • MDM2 oncogene E3 ubiquitin protein ligase
      • Mdm2 p53 E3 ubiquitin protein ligase homolog
      • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa
      • MDM2_HUMAN
      • MDM2BP
      • Mouse Double Minute 2
      • MTBP
      • Murine Double Minute Chromosome 2
      • Oncoprotein Mdm2
      • p53 Binding Protein Mdm2
      • p53-binding protein Mdm2
      • Ubiquitin protein ligase E3 Mdm2
      see all
    • Function

      E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.
    • Tissue specificity

      Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
    • Involvement in disease

      Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
    • Sequence similarities

      Belongs to the MDM2/MDM4 family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RING-type zinc finger.
      Contains 1 SWIB domain.
    • Domain

      Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
    • Post-translational
      modifications

      Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
      Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.
    • Cellular localization

      Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
    • Target information above from: UniProt accession Q00987 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MDM2 protein (ab82080)
      SDS-PAGE - Recombinant Human MDM2 protein (ab82080)

      SDS-PAGE analysis of Recombinant Human MDM2 protein (ab82080).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab82080? Please let us know so that we can cite the reference in this datasheet.

    ab82080 has been referenced in 1 publication.

    • Elshafey R  et al. Label-free impedimetric immunosensor for ultrasensitive detection of cancer marker Murine double minute 2 in brain tissue. Biosens Bioelectron 39:220-5 (2013). PubMed: 22898660

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Customer contacted us for MDM2 protein sequence ab82080

    Read More

    Abcam community

    Verified customer

    Asked on Jan 22 2013

    Answer


    The sequence for the MDM 2 protein (His tag) ab82080 is as follows:

    MAHHHHHHAS
    MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
    GLEHHHHHHHH

    Read More

    Abcam Scientific Support

    Answered on Jan 22 2013

    Question

    Dear Madam or Sir,
    I would like to know if the MDM2 protein (His tag) (ab82080) has enzymatic
    activity? Can I use it in an in vitro deubiquitination assay?
    Thanks a lot!
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2012

    Answer

    Thank you for your inquiry.

    I just went through our database and we do nothave any experimental data about the ligase activity ab82080 (MDM2).

    This protein together with p53 wt has beenused byseveral pharma companies and academic labssince 2005 and we have received no complains from these customers.

    The protein shall be active although we have little information about the applications customers used itfor.

    I am sorry I do not have a more precise answer and hope this information is nevertheless helpful.

    Read More

    Abcam Scientific Support

    Answered on Jun 11 2012

    Question

    What end of the protein is the His tag on? What is the exact sequence of this MDM2 protei

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2011

    Answer

    Thank you for your inquiry, and for your patience while I looked into this. The MDM2 protein has a 6x His tag on its N-terminus and 8 x Histag on its C-terminus. The protein sequence is given below: MAHHHHHHAS MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP GLEHHHHHHHH   Please do not hesitate to contact us if we can be of any further assistance to

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2011

    Question

    I would like to test ab82080 for functional studies involving protein-protein interactions.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2011

    Answer

    I am very pleased to hear you would like to accept our offer and test ab82080 in functional studies involving protein-protein interactions. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview for functional studies involving protein-protein interactions and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Oct 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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