Recombinant Human MED3 protein (denatured) (ab180327)

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Description

  • Product name

    Recombinant Human MED3 protein (denatured)
    See all MED3 proteins and peptides

  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    Q6P2C8

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMADVINVSVNLEAFSQAISAIQALRSS VSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLV GKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGL LNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMS IHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGK LDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLF QAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

    • Predicted molecular weight

      38 kDa including tags

    • Amino acids

      1 to 311

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_004260

Specifications

Our Abpromise guarantee covers the use of ab180327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as CRSP8

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 10% Glycerol, 2.4% Urea, 0.32% Tris HCl

General Info

  • Alternative names

    • Cofactor required for Sp1 transcriptional activation subunit 8
    • Cofactor required for Sp1 transcriptional activation subunit 8 (34kD)
    • Cofactor required for Sp1 transcriptional activation subunit 8 34kDa
    • CRAP 34
    • CRAP34
    • CRSP 34
    • CRSP 8
    • CRSP complex subunit 8
    • CRSP34
    • CRSP8
    • MED 27
    • Med27
    • MED27_HUMAN
    • Mediator complex subunit 27
    • Mediator of RNA polymerase II transcription subunit 27
    • MGC11274
    • P37 TRAP/SMCC/PC2 subunit
    • Transcriptional coactivator CRSP34
    • TRAP 37
    • TRAP37
    see all

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • Sequence similarities

    Belongs to the Mediator complex subunit 27 family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)
    SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)

    15% SDS-PAGE analysis of 3µg ab180327.

References

This product has been referenced in:

  • Ji E  et al. RNA Binding Protein HuR Promotes Autophagosome Formation by Regulating Expression of Autophagy-Related Proteins 5, 12, and 16 in Human Hepatocellular Carcinoma Cells. Mol Cell Biol 39:N/A (2019). Read more (PubMed: 30602494) »
See 1 Publication for this product

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