Recombinant Human MED3 protein (denatured) (ab180327)

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SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human MED3 protein (denatured)
      See all MED3 proteins and peptides

    • Purity

      > 80 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      Q6P2C8

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMADVINVSVNLEAFSQAISAIQALRSS VSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLV GKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGL LNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMS IHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGK LDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLF QAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

      • Predicted molecular weight

        38 kDa including tags

      • Amino acids

        1 to 311

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_004260

    Specifications

    Our Abpromise guarantee covers the use of ab180327 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as CRSP8

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

    General Info

    • Alternative names

      • Cofactor required for Sp1 transcriptional activation subunit 8
      • Cofactor required for Sp1 transcriptional activation subunit 8 (34kD)
      • Cofactor required for Sp1 transcriptional activation subunit 8 34kDa
      • CRAP 34
      • CRAP34
      • CRSP 34
      • CRSP 8
      • CRSP complex subunit 8
      • CRSP34
      • CRSP8
      • MED 27
      • Med27
      • MED27_HUMAN
      • Mediator complex subunit 27
      • Mediator of RNA polymerase II transcription subunit 27
      • MGC11274
      • P37 TRAP/SMCC/PC2 subunit
      • Transcriptional coactivator CRSP34
      • TRAP 37
      • TRAP37
      see all

    • Function

      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    • Sequence similarities

      Belongs to the Mediator complex subunit 27 family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)
      SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)

      15% SDS-PAGE analysis of 3µg ab180327.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab180327? Please let us know so that we can cite the reference in this datasheet.

    ab180327 has been referenced in 1 publication.

    • Ji E  et al. RNA Binding Protein HuR Promotes Autophagosome Formation by Regulating Expression of Autophagy-Related Proteins 5, 12, and 16 in Human Hepatocellular Carcinoma Cells. Mol Cell Biol 39:N/A (2019). PubMed: 30602494

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