Recombinant Human MEF2D protein (ab152520)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human MEF2D protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGRKKIQIQRITDERNRRVTFTKRKFGLMKKAYELSVLCDCEIALIIFNH SNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRKKGFNGCDSPE PDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNFAMPVTVPVSN QSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPA SAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAK SPPPPTHSTQLGAPSRKPDLRVITSQAGKGLMHHLNNAQRLGVSQSTHSL TTPVVSVATPSLLSQGLPFSSMPTAYNTDYQLTSAELSSLPAFSSPGGLS LGNVTAWQQPQQPQQPQQPQPPQQQPPQPQQPQPQQPQQPQQPPQQQSHL VPVSLSNLIPGSPLPHVGAALTVTTHPHISIKSEPVSPSRERSPAPPPPA VFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEG SAVKRMRLDTWTLK -
Predicted molecular weight
82 kDa including tags -
Amino acids
1 to 514 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab152520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
SDS-PAGE
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- DKFZp686I1536
- MADS box transcription factor 2 polypeptide D
- Mef2d
see all -
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. -
Sequence similarities
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
Developmental stage
Present in myotubes and also in undifferentiated myoblasts. -
Domain
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
Post-translational
modificationsPhosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
Cellular localization
Nucleus. Translocated by HDAC4 to nuclear dots. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human MEF2D protein (ab152520)12.5% SDS-PAGE analysis of ab152520 stained with Coomassie Blue.
Datasheets and documents
References
ab152520 has not yet been referenced specifically in any publications.
