Recombinant Human MEK1 protein (ab126919)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human MEK1 protein
See all MEK1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
71 kDa including tags -
Amino acids
1 to 393 -
Tags
GST tag N-Terminus
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Associated products
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Recombinant Protein
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab126919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
ab126919 (Human MEK1 full length protein) can be utilized as a substrate for the active protein kinase ab104027 (Active human MEKK1 protein fragment)
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.79% Tris-HCl buffer, 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
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Alternative names
- Dual specificity mitogen activated protein kinase kinase 1
- Dual specificity mitogen-activated protein kinase kinase 1
- ERK activator kinase 1
see all -
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
Tissue specificity
Widely expressed, with extremely low levels in brain. -
Involvement in disease
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126919 has not yet been referenced specifically in any publications.