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    recombinant-human-mek1-protein-ab63209.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Recombinant human MEK1 protein (ab63209)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human MEK1 protein (ab63209)
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
  • Functional Studies - Recombinant human MEK1 protein (ab63209)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human MEK1 protein
    See all MEK1 proteins and peptides
  • Biological activity

    Specific activity: 325 nmol/min/mg. Specific activity: 325 nmol/min/mg.

  • Purity

    > 95 % SDS-PAGE.
    Affinity purified.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      > 402 aa MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 60 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 120 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 180 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMPKK KPTPIQLNPA 240 PDGSAVNGTS SAETNLEALQ KKLEELELDE QQRKRLEAFL TQKQKVGELK DDDFEKISEL 300 GAGNGGVVFK VSHKPSGLVM ARKLIHLEIK PAIRNQIIRE LQVLHECNSP YIVGFYGAFY 360 SDGEISICME HMDGGSLDQV LKKAGRIPEQ ILGKVSIAVI KGLTYLREKH KIMHRDVKPS 420 NILVNSRGEI KLCDFGVSGQ LIDSMANSFV GTRSYMSPER LQGTHYSVQS DIWSMGLSLV 480 EMAVGRYPIP PPDAKELELM FGCQVEGDAA ETPPRPRTPG RPLSSYGMDS RPPMAIFELL 540 DYIVNEPPPK LPSGVFSLEF QDFVNKCLIK NPAERADLKQ LMVHAFIKRS DAEEVDFAGW 600 LCSTIGLNQP STPTHAAGV 619
    • Actual molecular weight

      72 kDa

Associated products

  • Related Products

    • Anti-MEK1 antibody [E342] (ab32091)
    • Anti-MEK1 antibody [Y77] (ab32576)
  • Substrate reagent

    • Recombinant Human ERK1 protein (ab126906)
    • Native Pig Myelin Basic Protein (ab64311)

Specifications

Our Abpromise guarantee covers the use of ab63209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab126906 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 1
    • Dual specificity mitogen-activated protein kinase kinase 1
    • ERK activator kinase 1
    • MAP kinase kinase 1
    • MAP kinase/Erk kinase 1
    • MAP2K1
    • MAPK/ERK kinase 1
    • MAPKK 1
    • MAPKK1
    • MEK 1
    • Mek1
    • MEKK1
    • Mitogen activated protein kinase kinase 1
    • MKK 1
    • MKK1
    • MP2K1_HUMAN
    • PRKMK1
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1)
    • Protein kinase mitogen activated, kinase 1
    • protein kinase mitogen-activated kinase 1
    see all
  • Function

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Tissue specificity

    Widely expressed, with extremely low levels in brain.
  • Involvement in disease

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Target information above from: UniProt accession Q02750 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human MEK1 protein (ab63209)
    Functional Studies - Recombinant human MEK1 protein (ab63209)
    The specific activity of MEK1 (ab63209) was determined to be 280 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS PAGE analysis of ab63209
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS PAGE analysis of ab63209
  • SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS-PAGE - Recombinant human MEK1 protein (ab63209)
    SDS Page analysis of ab63209
  • Functional Studies - Recombinant human MEK1 protein (ab63209)
    Functional Studies - Recombinant human MEK1 protein (ab63209)
    Specific activity standard curve of ab63209

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab63209? Please let us know so that we can cite the reference in this datasheet.

ab63209 has not yet been referenced specifically in any publications.

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