Recombinant human MEK4/MKK4 protein (Active) (ab268797)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human MEK4/MKK4 protein (Active)
See all MEK4/MKK4 proteins and peptides -
Biological activity
The specific activity of ab268797 was 4.6 nmol/min/mg in a kinase assay using unactive JNK1 protein as substrate.
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Purity
> 75 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
46 kDa by SDS-PSGE -
Tags
His tag N-Terminus -
Additional sequence information
NM_003010
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab268797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% DTT, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- c Jun N terminal kinase kinase 1
- C-JUN N-terminal kinase kinase 1
- Dual specificity mitogen activated protein kinase kinase 4
see all -
Function
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268797 has not yet been referenced specifically in any publications.