Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human MEK4/MKK4 protein (Active)
See all MEK4/MKK4 proteins and peptides
The specific activity of ab268797 was 4.6 nmol/min/mg in a kinase assay using unactive JNK1 protein as substrate.
Purity> 75 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight information46 kDa by SDS-PSGE
TagsHis tag N-Terminus
Additional sequence informationNM_003010
Our Abpromise guarantee covers the use of ab268797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% DTT, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- c Jun N terminal kinase kinase 1
- C-JUN N-terminal kinase kinase 1
- Dual specificity mitogen activated protein kinase kinase 4
FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268797 has not yet been referenced specifically in any publications.