Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      97 kDa including tags
    • Amino acids

      1 to 619

Associated products

Specifications

Our Abpromise guarantee covers the use of ab135009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 18 pmol/min/µg. Assay conditions: Enzyme reaction was conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30 °C. Enzyme activity was then measured.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.017% Potassium chloride, 0.06% DTT, 0.64% Tris HCl, 20% Glycerol, 0.65% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • M3K2_HUMAN
    • Map3k2
    • MAPK/ERK kinase kinase 2
    • MEK kinase 2
    • MEKK 2
    • MEKK2b
    • Mitogen activated protein kinase kinase kinase 2
    • Mitogen-activated protein kinase kinase kinase 2
    see all
  • Function

    Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus.
  • Information by UniProt

Images

  • 4-20% SDS PAGE analysis of ab135009 (0.7 μg).
    Lane 1: ab135009
    Lane 2: MW marker
  • Specific Activity of ab135009.

References

ab135009 has not yet been referenced specifically in any publications.

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