Description

  • Product name

    Recombinant human MEKK2 protein
    See all MEKK2 proteins and peptides
  • Biological activity

    Specific activity: 226nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab85299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab43623 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • M3K2_HUMAN
    • Map3k2
    • MAPK/ERK kinase kinase 2
    • MEK kinase 2
    • MEKK 2
    • MEKK2b
    • Mitogen activated protein kinase kinase kinase 2
    • Mitogen-activated protein kinase kinase kinase 2
    see all
  • Function

    Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Cytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus.
  • Information by UniProt

Images

  • The specific activity of MEKK2 (ab85299) was determined to be 239.3 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab85299
  • Specific activity of ab85299
  • SDS-Page Showing ab85299 at approximately 115 kDa

References

ab85299 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85299.
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