Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant human MELK protein
See all MELK proteins and peptides
The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
Purity> 85 % Densitometry.
Purity was determined to be >85% by densitometry. Affinity purified.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight61 kDa including tags
Amino acids1 to 340
Our Abpromise guarantee covers the use of ab102551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- HPK 38
FunctionPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificityExpressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102551 has not yet been referenced specifically in any publications.