Recombinant human MELK protein (Active) (ab204155)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human MELK protein (Active)
See all MELK proteins and peptides -
Biological activity
The specific activity of ab204155 was determined to be 210 nmol/min/mg.
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Purity
> 85 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSD LPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS QDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLID FGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLY VLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKK RISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQT MEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFT DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWT ESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPV NKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVIS PERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKG SA -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 550 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_014791.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab204155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine), 0.00385% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AI327312
- hMELK
- HPK 38
see all -
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human MELK protein (Active) (ab204155)The specific activity of MELK (ab204155) was determined to be 140 nmol/min/mg as per activity assay protocol and was equivalent to 175 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS PAGE analysis of ab204155 -
SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS PAGE analysis of ab204155 -
Functional Studies - Recombinant human MELK protein (Active) (ab204155)The specific activity of ab204155 was determined to be 210 nmol/min/mg.
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SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS-PAGE analysis of ab204155 which was determined to be >85% pure by densitometry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab204155 has not yet been referenced specifically in any publications.
