Recombinant human MELK protein (Active) (ab204155)
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- References
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Description
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Product name
Recombinant human MELK protein (Active)
See all MELK proteins and peptides -
Biological activity
The specific activity of ab204155 was determined to be 210 nmol/min/mg.
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Purity
> 85 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSD LPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS QDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLID FGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLY VLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKK RISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQT MEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFT DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWT ESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPV NKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVIS PERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKG SA -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 550 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_014791.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab204155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 25% Glycerol, 0.00385% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AI327312
- hMELK
- HPK 38
see all -
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human MELK protein (Active) (ab204155)The specific activity of MELK (ab204155) was determined to be 140 nmol/min/mg as per activity assay protocol and was equivalent to 175 nmol/min/mg as per radiometric assay -
SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS PAGE analysis of ab204155 -
SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS PAGE analysis of ab204155 -
Functional Studies - Recombinant human MELK protein (Active) (ab204155)The specific activity of ab204155 was determined to be 210 nmol/min/mg.
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SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)SDS-PAGE analysis of ab204155 which was determined to be >85% pure by densitometry.
Datasheets and documents
References
ab204155 has not yet been referenced specifically in any publications.
