Recombinant human MELK protein (Active) (ab204155)

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Description

  • Product name

    Recombinant human MELK protein (Active)
    See all MELK proteins and peptides

  • Biological activity

    The specific activity of ab204155 was determined to be 210 nmol/min/mg.

  • Purity

    > 85 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q14680

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSD LPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS QDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLID FGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLY VLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKK RISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQT MEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFT DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWT ESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPV NKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVIS PERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKG SA

    • Predicted molecular weight

      88 kDa including tags

    • Amino acids

      1 to 550

    • Tags

      GST tag N-Terminus

    • Additional sequence information

      NM_014791.

Specifications

Our Abpromise guarantee covers the use of ab204155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 25% Glycerol, 0.00385% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • AI327312
    • hMELK
    • HPK 38
    • hPK38
    • KIAA0175
    • Likely ortholog of maternal embryonic leucine zipper kinase
    • Maternal embryonic leucine zipper kinase
    • MELK
    • MELK_HUMAN
    • mKIAA0175
    • MPK38
    • OTTHUMP00000021377
    • OTTHUMP00000046113
    • pEg3 kinase
    • Protein kinase Eg3
    • Protein kinase PK38
    • RP23 382O11.1
    • Tyrosine protein kinase MELK
    see all

  • Function

    Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.

  • Tissue specificity

    Expressed in placenta, kidney, thymus, testis, ovary and intestine.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human MELK protein (Active) (ab204155)
    Functional Studies - Recombinant human MELK protein (Active) (ab204155)
    The specific activity of MELK (ab204155) was determined to be 140 nmol/min/mg as per activity assay protocol and was equivalent to 175 nmol/min/mg as per radiometric assay
  • SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
    SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
    SDS PAGE analysis of ab204155
  • SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
    SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
    SDS PAGE analysis of ab204155
  • Functional Studies - Recombinant human MELK protein (Active) (ab204155)
    Functional Studies - Recombinant human MELK protein (Active) (ab204155)

    The specific activity of ab204155 was determined to be 210 nmol/min/mg.

  • SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
    SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)

    SDS-PAGE analysis of ab204155 which was determined to be >85% pure by densitometry.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab204155? Please let us know so that we can cite the reference in this datasheet.

ab204155 has not yet been referenced specifically in any publications.

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