Recombinant Human Mesothelin protein (ab205217)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Human Mesothelin protein
See all Mesothelin proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab205217 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGEFLAGETGQEAAPLDGVLANPPN ISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHR LSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAP ERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPR LVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQ PIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRREVEKTACPSGKK APEIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLD ELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEM SPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSI WAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTED LKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHR PVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS -
Predicted molecular weight
65 kDa including tags -
Amino acids
37 to 598 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 37 to 598. The signal peptide and propeptide are not included. With 29 aa N-terminal T7 / His / TEV cleavage site tag (NP_005814.2).
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab205217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, Sucrose and DTT.
General Info
-
Alternative names
- CAK 1
- CAK1
- CAK1 antigen
see all -
Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab205217 has not yet been referenced specifically in any publications.