Recombinant Human Mesothelin protein (ab205217)

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Description

  • Product name

    Recombinant Human Mesothelin protein
    See all Mesothelin proteins and peptides

  • Purity

    > 90 % SDS-PAGE.
    ab205217 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.

  • Expression system

    Escherichia coli

  • Accession

    Q13421-3

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MASMTGGQQMGRGHHHHHHENLYFQGGEFLAGETGQEAAPLDGVLANPPN ISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHR LSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAP ERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPR LVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQ PIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRREVEKTACPSGKK APEIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLD ELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEM SPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSI WAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTED LKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHR PVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS

    • Predicted molecular weight

      65 kDa including tags

    • Amino acids

      37 to 598

    • Tags

      His-T7 tag N-Terminus

    • Additional sequence information

      This product is the mature full length protein from aa 37 to 598. The signal peptide and propeptide are not included. With 29 aa N-terminal T7 / His / TEV cleavage site tag (NP_005814.2).

Specifications

Our Abpromise guarantee covers the use of ab205217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C.

    pH: 8.00
    Constituent: 0.32% Tris HCl

    Contains NaCl, KCl, EDTA, Sucrose and DTT.

General Info

  • Alternative names

    • CAK 1
    • CAK1
    • CAK1 antigen
    • cleaved form
    • Megakaryocyte potentiating factor
    • Mesothelin
    • Mesothelin isoform 1 precursor
    • MPF
    • Msln
    • MSLN_HUMAN
    • Pre pro megakaryocyte potentiating factor
    • Pre-pro-megakaryocyte-potentiating factor
    • SMR
    • SMRP
    • Soluble MPF mesothelin related protein
    see all

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

  • Sequence similarities

    Belongs to the mesothelin family.

  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt

References

ab205217 has not yet been referenced specifically in any publications.

Publishing research using ab205217? Please let us know so that we can cite the reference in this datasheet.

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