Recombinant Human Mesothelin protein (ab245958)
Key features and details
- Expression system: CHO cells
- Endotoxin level: < 1.000 Eu/µg
- Suitable for: SDS-PAGE, Functional Studies, HPLC
Description
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Product name
Recombinant Human Mesothelin protein
See all Mesothelin proteins and peptides -
Endotoxin level
< 1.000 Eu/µg -
Expression system
CHO cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELA VALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQA CTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALG GLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGP PSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPER -
Predicted molecular weight
27 kDa -
Amino acids
37 to 286 -
Additional sequence information
Corresponding to aa 37-286 Megakaryocyte-potentiating factor chain.
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Associated products
Specifications
Our Abpromise guarantee covers the use of ab245958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
HPLC
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Form
Lyophilized -
Additional notes
As a result of glycosylation, ab245958 migrates with an apparent molecular mass of approximately 32-33 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
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ReconstitutionReconstitute in water to 0.1 - 1.0 mg/ml.
General Info
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Alternative names
- CAK 1
- CAK1
- CAK1 antigen
see all -
Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab245958 has not yet been referenced specifically in any publications.