Recombinant Human Mesothelin protein (ab245958)

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Key features and details

  • Expression system: CHO cells
  • Endotoxin level: < 1.000 Eu/µg
  • Suitable for: SDS-PAGE, Functional Studies, HPLC

Description

  • Product name

    Recombinant Human Mesothelin protein
    See all Mesothelin proteins and peptides

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    CHO cells

  • Accession

    Q13421

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELA VALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQA CTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALG GLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGP PSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPER

    • Predicted molecular weight

      27 kDa

    • Amino acids

      37 to 286

    • Additional sequence information

      Corresponding to aa 37-286 Megakaryocyte-potentiating factor chain.

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab245958 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      HPLC

    • Form

      Lyophilized

    • Additional notes

      As a result of glycosylation, ab245958 migrates with an apparent molecular mass of approximately 32-33 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: 0.16% Sodium phosphate

    • Reconstitution
      Reconstitute in water to 0.1 - 1.0 mg/ml.

    General Info

    • Alternative names

      • CAK 1
      • CAK1
      • CAK1 antigen
      • cleaved form
      • Megakaryocyte potentiating factor
      • Mesothelin
      • Mesothelin isoform 1 precursor
      • MPF
      • Msln
      • MSLN_HUMAN
      • Pre pro megakaryocyte potentiating factor
      • Pre-pro-megakaryocyte-potentiating factor
      • SMR
      • SMRP
      • Soluble MPF mesothelin related protein
      see all

    • Function

      Membrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

    • Tissue specificity

      Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

    • Involvement in disease

      Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

    • Sequence similarities

      Belongs to the mesothelin family.

    • Post-translational
      modifications

      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

    • Cellular localization

      Secreted and Cell membrane. Golgi apparatus.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab245958? Please let us know so that we can cite the reference in this datasheet.

    ab245958 has not yet been referenced specifically in any publications.

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