Description

  • Product name

    Recombinant human Mesothelin protein (Active)
    See all Mesothelin proteins and peptides
  • Biological activity

    Assay #1: Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind ab246135 with a linear range of 0.039-0.625 μg/mL (QC tested).

    Assay #2: Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind ab246135 with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFT YEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFY PGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNM NGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVA EVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLD
    • Predicted molecular weight

      60 kDa including tags
    • Amino acids

      296 to 580
    • Tags

      His tag N-Terminus
    • Additional sequence information

      This protein carries a GFP tag at the N-terminus and a polyhistidine tag at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab246135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    0.22 µm filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • CAK 1
    • CAK1
    • CAK1 antigen
    • cleaved form
    • Megakaryocyte potentiating factor
    • Mesothelin
    • Mesothelin isoform 1 precursor
    • MPF
    • Msln
    • MSLN_HUMAN
    • Pre pro megakaryocyte potentiating factor
    • Pre-pro-megakaryocyte-potentiating factor
    • SMR
    • SMRP
    • Soluble MPF mesothelin related protein
    see all
  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities

    Belongs to the mesothelin family.
  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt

Images

  • FACS bioactivity analysis of 2 x 105 MSLN-CAR-293 cells stained with 100ul of 3 μg/mL of ab246135. GFP was used as negative control.

  • Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind ab246135 with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200).

  • Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind ab246135 with a linear range of 0.039-0.625 μg/mL.

  • SDS-page analysis of ab246135 under reducing conditions. The gel was stained overnight with coomassie blue. The protein migrates as 70 kDa under reducing conditions due to glycosylation.

References

ab246135 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab246135.
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