Recombinant Human Metallothionein protein (Tagged) (ab112324)

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Description

  • Product name

    Recombinant Human Metallothionein protein (Tagged)

  • Expression system

    Wheat germ

  • Accession

    P02795

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MDPNCSCAAGDSCTCAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCIC KGASDKCSCCA

    • Predicted molecular weight

      33 kDa including tags

    • Amino acids

      1 to 61

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab112324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • Form

    Liquid

  • Additional notes

    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione

General Info

  • Alternative names

    • CES 1
    • CES1
    • Metallothionein 1A
    • Metallothionein 1S
    • Metallothionein 2
    • Metallothionein 2A
    • Metallothionein IA
    • Metallothionein II
    • Metallothionein-1A
    • Metallothionein-IA
    • Metallothionein2
    • MGC32848
    • MT 1A
    • MT 2
    • MT 2A
    • MT IA
    • MT II
    • MT-1A
    • MT-IA
    • MT1
    • MT1A
    • MT1A_HUMAN
    • MT1S
    • MT2
    • MT2A
    • MTC
    see all

  • Function

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

  • Sequence similarities

    Belongs to the metallothionein superfamily. Type 1 family.

  • Domain

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Metallothionein protein (Tagged) (ab112324)
    SDS-PAGE - Recombinant Human Metallothionein protein (Tagged) (ab112324)
    ab112324 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab112324 has not yet been referenced specifically in any publications.

Publishing research using ab112324? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Is there a protease cleavage site present between the GST and metallotheonein?

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Answer

The tag is at the N-terminus and it could be cleavaged from the fusion protein by PreScission Protease (Amersham Biosciences).

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Question

To whom it may concern,

I wish to seek some proteins
Metallothionein 1X
Metallothionein 2A

Any help would be greatly appreciated

Many thanks,

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Answer

Thank you for your enquiry.

I can confirm we do have one Matallothionein protein available in the catalog:

ab112324
https://www.abcam.com/index.html?datasheet=112324 (or use the following: https://www.abcam.com/index.html?datasheet=112324).

I have confirmed with the originator that this is Metallothionein 2A protein (GeneID: 4502).

I am sorry we do not have Metallothionein 1X.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Would like MSDS for this protein.

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Answer

Thanks for your call today and for your patience. Please find the MSDS attached to this email. Let me know if you have any questions or if there is anything else that we can do for you.

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Question

Please send MSDS sheet.

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Answer

Thank you for calling Abcam. Please find attached the MSDS for ab112324. If there is anything else I can help you with, please let me kn

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