Recombinant Human Metallothionein protein (Tagged) (ab112324)

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SDS-PAGE - Recombinant Human Metallothionein protein (Tagged) (ab112324)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE, ELISA, WB

    Description

    • Product name

      Recombinant Human Metallothionein protein (Tagged)

    • Expression system

      Wheat germ

    • Accession

      P02795

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MDPNCSCAAGDSCTCAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCIC KGASDKCSCCA

      • Predicted molecular weight

        33 kDa including tags

      • Amino acids

        1 to 61

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab112324 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      ELISA

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • CES 1
      • CES1
      • Metallothionein 1A
      • Metallothionein 1S
      • Metallothionein 2
      • Metallothionein 2A
      • Metallothionein IA
      • Metallothionein II
      • Metallothionein-1A
      • Metallothionein-IA
      • Metallothionein2
      • MGC32848
      • MT 1A
      • MT 2
      • MT 2A
      • MT IA
      • MT II
      • MT-1A
      • MT-IA
      • MT1
      • MT1A
      • MT1A_HUMAN
      • MT1S
      • MT2
      • MT2A
      • MTC
      see all

    • Function

      Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

    • Sequence similarities

      Belongs to the metallothionein superfamily. Type 1 family.

    • Domain

      Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Metallothionein protein (Tagged) (ab112324)
      SDS-PAGE - Recombinant Human Metallothionein protein (Tagged) (ab112324)
      ab112324 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab112324? Please let us know so that we can cite the reference in this datasheet.

    ab112324 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    Is there a protease cleavage site present between the GST and metallotheonein?

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    Answer

    The tag is at the N-terminus and it could be cleavaged from the fusion protein by PreScission Protease (Amersham Biosciences).

    Read More

    Question

    To whom it may concern,

    I wish to seek some proteins
    Metallothionein 1X
    Metallothionein 2A

    Any help would be greatly appreciated

    Many thanks,

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm we do have one Matallothionein protein available in the catalog:

    ab112324
    https://www.abcam.com/Recombinant-Human-Metallothionein-protein-Tagged-ab112324.html (or use the following: https://www.abcam.com/Recombinant-Human-Metallothionein-protein-Tagged-ab112324.html).

    I have confirmed with the originator that this is Metallothionein 2A protein (GeneID: 4502).

    I am sorry we do not have Metallothionein 1X.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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    Question

    Would like MSDS for this protein.

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    Answer

    Thanks for your call today and for your patience. Please find the MSDS attached to this email. Let me know if you have any questions or if there is anything else that we can do for you.

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    Question

    Please send MSDS sheet.

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    Answer

    Thank you for calling Abcam. Please find attached the MSDS for ab112324. If there is anything else I can help you with, please let me kn

    Read More

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