Recombinant Human Methionine Aminopeptidase 2/p67 protein (His tag) (ab232796)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Methionine Aminopeptidase 2/p67 protein (His tag)
See all Methionine Aminopeptidase 2/p67 proteins and peptides -
Purity
> 85 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAA GEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKK KKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTT SEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLE DCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKID FGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVG EAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGE ATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKH LLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIK GSYTAQFEHTILLRPTCKEVVSRGDDY -
Predicted molecular weight
53 kDa -
Amino acids
2 to 478 -
Tags
His tag N-Terminus -
Additional sequence information
Myc tag C-Terminus
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Associated products
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Related Products
- Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523)
- Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)
- Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [9E10] (ab32)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
Specifications
Our Abpromise guarantee covers the use of ab232796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as Methionine Aminopeptidase 2
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- A930035J23Rik
- AI047573
- AL024412
see all -
Function
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
Sequence similarities
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. -
Post-translational
modificationsContains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. -
Cellular localization
Cytoplasm. About 30% of expressed METAP2 associates with polysomes. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232796 has not yet been referenced specifically in any publications.