Key features and details
- Expression system: Baculovirus
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Methionine Aminopeptidase 2/p67 protein (His tag)
See all Methionine Aminopeptidase 2/p67 proteins and peptides
Purity> 85 % SDS-PAGE.
Protein lengthFull length protein
SequenceAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAA GEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKK KKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTT SEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLE DCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKID FGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVG EAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGE ATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKH LLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIK GSYTAQFEHTILLRPTCKEVVSRGDDY
Predicted molecular weight53 kDa
Amino acids2 to 478
TagsHis tag N-Terminus
Additional sequence informationMyc tag C-Terminus
- Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523)
- Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)
- Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [9E10] (ab32)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
Our Abpromise guarantee covers the use of ab232796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Methionine Aminopeptidase 2
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
FunctionCotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Sequence similaritiesBelongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
modificationsContains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
Cellular localizationCytoplasm. About 30% of expressed METAP2 associates with polysomes.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232796 has not yet been referenced specifically in any publications.