Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
    • Molecular weight
      25 kDa including tags
    • Amino acids
      1 to 207

Specifications

Our Abpromise guarantee covers the use of ab136378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % Densitometry.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • 6 O methylguanine DNA methyltransferase
    • 6-O-methylguanine-DNA methyltransferase
    • Agat
    • AGT
    • AI267024
    • EC 2.1.1.63
    • Methylated DNA protein cysteine methyltransferase
    • Methylated-DNA--protein-cysteine methyltransferase
    • Methylguanine DNA methyltransferase
    • MGC107020
    • MGMT
    • MGMT_HUMAN
    • O 6 methylguanine DNA alkyltransferase
    • O 6 methylguanine DNA methyltransferase
    • O-6-methylguanine-DNA methyltransferase
    • O-6-methylguanine-DNA-alkyltransferase
    see all
  • Function
    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
  • Sequence similarities
    Belongs to the MGMT family.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE using ab136378

References

ab136378 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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