Recombinant Human MGRN1 protein (ab167954)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human MGRN1 protein
See all MGRN1 proteins and peptides -
Purity
> 90 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEG YLFGENMDLNFLGSRPVQFPYVTPAPHEPVKTLRSLVNIRKDSLRLVRYK DDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASEEFLNGRAVYSP KSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVI QAVVDEGDVVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQ EIYGIENKNNQETKPSDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTS CADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEH DEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAV SPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKA PDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITE EVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAH GLATTSPTWPPLGGPSPDPSAAELTPL -
Predicted molecular weight
96 kDa including tags -
Amino acids
2 to 576 -
Tags
proprietary tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
Previously labelled as RNF156.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol, 0.29% Sodium chloride
General Info
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Alternative names
- E3 ubiquitin-protein ligase MGRN1
- KIAA0544
- Mahogunin ring finger 1
see all -
Function
E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The RING finger is required for ubiquitin ligase activity. -
Post-translational
modificationsAutoubiquitinated in vitro. -
Cellular localization
Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab167954 has not yet been referenced specifically in any publications.