Recombinant Human MGRN1 protein (ab167954)

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SDS-PAGE - Recombinant Human MGRN1 protein (ab167954)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 90% Densitometry
    • Tags: proprietary tag N-Terminus
    • Suitable for: SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human MGRN1 protein
      See all MGRN1 proteins and peptides

    • Purity

      > 90 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      O60291-2

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        GSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEG YLFGENMDLNFLGSRPVQFPYVTPAPHEPVKTLRSLVNIRKDSLRLVRYK DDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASEEFLNGRAVYSP KSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVI QAVVDEGDVVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQ EIYGIENKNNQETKPSDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTS CADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEH DEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAV SPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKA PDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITE EVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAH GLATTSPTWPPLGGPSPDPSAAELTPL

      • Predicted molecular weight

        96 kDa including tags

      • Amino acids

        2 to 576

      • Tags

        proprietary tag N-Terminus

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab167954 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

      Previously labelled as RNF156. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol, 0.29% Sodium chloride

    General Info

    • Alternative names

      • E3 ubiquitin-protein ligase MGRN1
      • KIAA0544
      • Mahogunin ring finger 1
      • Mahogunin RING finger protein 1
      • MGRN 1
      • Mgrn1
      • MGRN1_HUMAN
      • Probable E3 ubiquitin protein ligase MGRN1
      • RING finger protein 156
      • RNF 156
      • RNF156
      see all

    • Function

      E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Contains 1 RING-type zinc finger.

    • Domain

      The RING finger is required for ubiquitin ligase activity.

    • Post-translational
      modifications

      Autoubiquitinated in vitro.

    • Cellular localization

      Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MGRN1 protein (ab167954)
      SDS-PAGE - Recombinant Human MGRN1 protein (ab167954)
      SDS-PAGE analysis of ab167954.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167954? Please let us know so that we can cite the reference in this datasheet.

    ab167954 has not yet been referenced specifically in any publications.

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