Recombinant Human MGRN1 protein (ab167954)

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Description

  • Product name

    Recombinant Human MGRN1 protein
    See all MGRN1 proteins and peptides

  • Purity

    > 90 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    O60291-2

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      GSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEG YLFGENMDLNFLGSRPVQFPYVTPAPHEPVKTLRSLVNIRKDSLRLVRYK DDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASEEFLNGRAVYSP KSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVI QAVVDEGDVVEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQ EIYGIENKNNQETKPSDDENSDNSNECVVCLSDLRDTLILPCRHLCLCTS CADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLEH DEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAV SPAIPSAPLYEEITYSGISDGLSQASCPLAAIDHILDSSRQKGRPQSKA PDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRESSSPESFITE EVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAH GLATTSPTWPPLGGPSPDPSAAELTPL

    • Predicted molecular weight

      96 kDa including tags

    • Amino acids

      2 to 576

    • Tags

      proprietary tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab167954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    Previously labelled as RNF156. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.004% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • E3 ubiquitin-protein ligase MGRN1
    • KIAA0544
    • Mahogunin ring finger 1
    • Mahogunin RING finger protein 1
    • MGRN 1
    • Mgrn1
    • MGRN1_HUMAN
    • Probable E3 ubiquitin protein ligase MGRN1
    • RING finger protein 156
    • RNF 156
    • RNF156
    see all

  • Function

    E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 RING-type zinc finger.

  • Domain

    The RING finger is required for ubiquitin ligase activity.

  • Post-translational
    modifications

    Autoubiquitinated in vitro.

  • Cellular localization

    Cytoplasm > cytosol. Cell membrane; Early endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human MGRN1 protein (ab167954)
    SDS-PAGE - Recombinant Human MGRN1 protein (ab167954)
    SDS-PAGE analysis of ab167954.

References

ab167954 has not yet been referenced specifically in any publications.

Publishing research using ab167954? Please let us know so that we can cite the reference in this datasheet.

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