Recombinant Human MIIP protein (ab163735)
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- References
- Protocols
Description
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Product name
Recombinant Human MIIP protein -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MVEAEELAQLRLLNLELLRQLWVGQDAVRRSVARAASESSLESSSSYNSE TPSTPETSSTSLSTSCPRGRSSVWGPPDACRGDLRDVARSGVASLPPANC QHQESLGRPRPHSAPSLGTSSLRDPEPSGRLGDPGPQEAQTSRSILAQQS KLSKPRVTFSEESAVPERSWRLRPYLGYDWIAGSLDTSSSITSQPEAFFS KLQEFRETNKEECICSHPEPQLPGLRESSGSGVEEDHECVYCYRVNRRLF PVPVDPGTPCRLCRTPRDQQGPGTLAQPAHVRVSIPLSILEPPHRYHIHR RKSFDASDTLALPRHCLLGWDIFPPKSEKSSAPRNLDLWSSVSAEAQHQK LSGTSSPFHPASPMQMLPPTPTWSVPQVPRPHVPRQKP -
Amino acids
1 to 388 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab163735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
Protein concentration is above or equal to 0.05 mg/ml. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- FLJ12438
- FLJ38609
- IGFBP 2 binding protein
see all -
Relevance
MIIP has 3 SEG (segments of low compositional complexity) domains, an RGD motif, and several potential phosphorylation sites. The C-terminal region of IGFBP2 interacts with a central 44-amino acid sequence of MIIP. MIIP inhibits glioma cells invasion and down-regulates adhesion- and motility-associated genes such as NFKB2 and ICAM1. It exhibits opposing effects to IGFBP2 on cell invasion. There are 2 named isoforms due to alternative splicing. Isoform 1 is expressed in brain but underexpressed in glioma tissues, at protein level. Isoform 2 is not detected in normal organs, but is expressed in gliomas with increasing levels with glioma progression. On the contrary, at protein level, isoform 2 is not detected in gliomas, suggesting that this isoform is unstable in glioma cells. Isoform 2 is degraded by the ubiquitin-proteasome pathway.
Images
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SDS-PAGE - Recombinant Human MIIP protein (ab163735)ab163735 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Datasheets and documents
References
ab163735 has not yet been referenced specifically in any publications.
